从纯化的牛心细胞色素c氧化酶制备二维阵列。

Preparation of two-dimensional arrays from purified beef heart cytochrome c oxidase.

作者信息

Fuller S D, Capaldi R A, Henderson R

出版信息

Biochemistry. 1982 May 11;21(10):2525-9. doi: 10.1021/bi00539a036.

Abstract

A method for preparing two-dimensional crystals from highly purified beef heart cytochrome c oxidase is described. This involves mixing the enzyme with phosphatidylcholine and then extracting excess lipid with deoxycholate. The reconstituted crystals show PI symmetry. Alternating rows of monomers are more closely packed (along the b axis) than in the previously described P121 crystals. However, the monomer structure in projection is the same in the two crystal forms. The P121 crystal form has been reacted with trypsin. This treatment did not alter the crystals but removed most of the impurities present in these cytochrome c oxidase preparation of low purity.

摘要

本文描述了一种从高度纯化的牛心细胞色素c氧化酶制备二维晶体的方法。这包括将该酶与磷脂酰胆碱混合，然后用脱氧胆酸盐提取过量的脂质。重构后的晶体呈现PI对称性。与先前描述的P121晶体相比，交替排列的单体行（沿b轴）堆积得更紧密。然而，两种晶体形式在投影中的单体结构是相同的。P121晶体形式已用胰蛋白酶处理。这种处理没有改变晶体，但去除了这些低纯度细胞色素c氧化酶制剂中存在的大部分杂质。