交感神经元中烟碱型乙酰胆碱受体通道的两种活动模式。

Two modes of activity of nicotinic acetylcholine receptor channels in sympathetic neurons.

作者信息

Skok V J, Selyanko A A, Derkach V A

出版信息

Brain Res. 1982 Apr 29;238(2):480-3. doi: 10.1016/0006-8993(82)90127-5.

Abstract

Spectral analysis of acetylcholine (ACh) noise was performed in voltage-clamped neurons of the isolated rabbit superior cervical ganglion at 34-37 degrees C and at membrane potential--80 mV. Two modes of activity were found in the ionic channels of nicotinic ACh receptors, with mean channel life-times of for fast-operating channels and for slow-operating channels. Excitatory postsynaptic current (EPSC) decays exponentially with a time constant which is very close to , indicating that the slow-operating channel activity determines the duration of EPSC. The mean value of conductance of single nicotinic ACh-receptor channel is 36 more or less 3 pS.

摘要

在34 - 37摄氏度和膜电位为 - 80 mV的条件下，对分离的兔颈上神经节的电压钳制神经元进行了乙酰胆碱（ACh）噪声的频谱分析。在烟碱型ACh受体的离子通道中发现了两种活动模式，快速运作通道的平均通道寿命为，慢速运作通道的平均通道寿命为。兴奋性突触后电流（EPSC）呈指数衰减，时间常数非常接近，这表明慢速运作通道的活动决定了EPSC的持续时间。单个烟碱型ACh受体通道的电导平均值约为36 ± 3 pS。