Yamamoto T, Asano T, Mori A, Kumai C, Okumura M, Soji T
Endocrinol Jpn. 1981 Oct;28(5):563-7. doi: 10.1507/endocrj1954.28.563.
The isolation of pancreatic islets from collagenase-digested Wistar rat pancreas was shown by the sedimentation method at a unit gravity using Percoll solution with a density of 1.041 g/ml. The density of digested exocrine tissues was in the range of 1.013-1.041 g/ml, while that of purely isolated islets was in the narrow range of 1.066-1.075 g/ml. More than a hundred islets were obtained freely from each rat pancreas without any gross contamination of digested exocrine tissues. A significant increase was observed in glucose-stimulated insulin release from islet isolated with Percoll in the same pattern as that without Percoll. In addition to the well preserved morphology and function of pancreatic islets isolated by Percoll, the simplicity of the technique strongly commends the usefullness of this method.
采用密度为1.041 g/ml的Percoll溶液，通过单位重力沉降法从经胶原酶消化的Wistar大鼠胰腺中分离胰岛。消化后的外分泌组织密度在1.013 - 1.041 g/ml范围内，而纯分离的胰岛密度在1.066 - 1.075 g/ml的狭窄范围内。从每只大鼠胰腺中可轻松获得一百多个胰岛，且无消化后的外分泌组织的明显污染。与未使用Percoll分离的胰岛一样，观察到用Percoll分离的胰岛在葡萄糖刺激下胰岛素释放显著增加。除了通过Percoll分离的胰岛形态和功能保存良好外，该技术的简单性也强烈表明了该方法的实用性。
