Kitagawa T, Tanimori H, Yoshida K, Miura T, Fujiwara K
J Biochem. 1982 May;91(5):1601-5. doi: 10.1093/oxfordjournals.jbchem.a133851.
Precise immunological recognition of anti-viomycin antiserum at detailed parts in the structure of viomycin was studied by cross reactivities of the antiserum to viomycin and its ten analogs using an enzyme immunoassay of viomycin. The antiserum clearly recognized all minor modifications in the sixteen membered ring of viomycin, indicating that the antiserum clearly recognizes the whole structure of the sixteen membered ring. Recognition of the antiserum on the beta-lysine terminus was also examined showing that the antiserum was also recognized on this part. Thus, the anti-viomycin antiserum was deduced to recognize the whole structure of viomycin, from which the deduction was made that the anti-viomycin antibodies in the antiserum must possess cavities fitting the whole structure of viomycin. The crystal dimensions of viomycin are 13 A in length, 8 A in width, and 7 A in depth. Thus, the high dimensional structure of the binding sites of the anti-viomycin antibodies was deduced to possess cavities of a similar size to that of viomycin.
通过使用紫霉素酶免疫测定法，研究抗紫霉素抗血清与紫霉素及其十种类似物的交叉反应，以精确免疫识别紫霉素结构中各个部分的抗紫霉素抗血清。该抗血清能够清晰识别紫霉素十六元环上的所有微小修饰，表明该抗血清能够清晰识别十六元环的整体结构。还检测了抗血清对β - 赖氨酸末端的识别情况，结果显示抗血清也能识别这一部分。因此，推断抗紫霉素抗血清能够识别紫霉素的整体结构，据此推测抗血清中的抗紫霉素抗体必定具有与紫霉素整体结构相适配的腔隙。紫霉素的晶体尺寸为长13埃、宽8埃、深7埃。因此，推断抗紫霉素抗体结合位点的高维结构具有与紫霉素大小相似的腔隙。
