喂食过量亮氨酸饮食以及低蛋白或高蛋白饮食的大鼠肝脏中的烟酰胺腺嘌呤二核苷酸糖水解酶活性。

NAD glycohydrolase activity in the liver of rats fed on excess leucine diet and low or high protein diet.

作者信息

Kubota H, Nomura K, Yamada O, Shin M, Sano K, Umezawa C

出版信息

J Nutr Sci Vitaminol (Tokyo). 1982 Feb;28(1):57-66. doi: 10.3177/jnsv.28.57.

DOI:10.3177/jnsv.28.57

Abstract

In rats fed for 2 weeks on a 10% casein diet supplemented with 5% leucine, the hepatic NAD content was lowered when comparing with the control rats pair-fed on the 10% casein diet. The hepatic NAD glycohydrolase [EC 3.2.2.5] activity increased by 25% in the leucine-supplemented diet-fed rats and by 40% in the nicotinic acid-free diet-fed rats. The hepatic NAD glycohydrolase activity in the rats fed on the nonprotein diet freely for 1 week was elevated by 60% compared to the rats fed on the 18% casein diet. The hepatic NAD content in the former animals was significantly lower than that in the latter. The 70% casein diet caused changes in neither hepatic NAD content nor NAD glycohydrolase activity. These results indicated an inverse relationship between hepatic NAD content and NAD glycohydrolase activity.

摘要

在以含5%亮氨酸的10%酪蛋白饮食喂养2周的大鼠中，与以10%酪蛋白饮食配对喂养的对照大鼠相比，肝脏中烟酰胺腺嘌呤二核苷酸（NAD）含量降低。在添加亮氨酸饮食喂养的大鼠中，肝脏烟酰胺腺嘌呤二核苷酸糖水解酶[EC 3.2.2.5]活性增加了25%，在无烟酸饮食喂养的大鼠中增加了40%。自由采食非蛋白饮食1周的大鼠肝脏烟酰胺腺嘌呤二核苷酸糖水解酶活性比采食18%酪蛋白饮食的大鼠升高了60%。前一组动物的肝脏NAD含量明显低于后一组。70%酪蛋白饮食对肝脏NAD含量和烟酰胺腺嘌呤二核苷酸糖水解酶活性均无影响。这些结果表明肝脏NAD含量与烟酰胺腺嘌呤二核苷酸糖水解酶活性之间呈负相关。