Rotella C M, Tanini A, Zonefrati R, Toccafondi R
J Recept Res. 1981;2(4):367-82. doi: 10.3109/107998981809038873.
In order to verify the existence of a "short-loop" negative feedback between iodothyronines and adenylate cyclase system of human thyroid, we have studied the effect of preincubation with iodothyronines, iodotyrosines, iodothyronine analogues and iodide on TSH-induced cAMP cellular accumulation in normal human thyroid cells in primary culture. Iodide did not produce an inhibitory effect on TSH-dependent adenylate cyclase system both in normal human thyroid plasma membranes and cultured cells. Iodothyronines at a 30-40 microM concentration did not inhibit the TSH-dependent adenylate cyclase activity of human thyroid plasma membranes; however at a 1 microM concentration they were able to inhibit the TSH-dependent cAMP accumulation by cultured cells. Preincubation with iodotyrosines and iodothyronine analogues failed to inhibit the TSH-responsive cAMP accumulation in human thyroid cultured cells.
为了验证甲状腺素与人甲状腺腺苷酸环化酶系统之间“短环”负反馈的存在，我们研究了用甲状腺素、碘酪氨酸、甲状腺素类似物和碘化物预孵育对原代培养的正常人甲状腺细胞中促甲状腺激素（TSH）诱导的环磷酸腺苷（cAMP）细胞内积累的影响。碘在正常人甲状腺质膜和培养细胞中均未对TSH依赖性腺苷酸环化酶系统产生抑制作用。浓度为30 - 40微摩尔的甲状腺素并未抑制人甲状腺质膜中TSH依赖性腺苷酸环化酶活性；然而，浓度为1微摩尔时，它们能够抑制培养细胞中TSH依赖性cAMP的积累。用碘酪氨酸和甲状腺素类似物预孵育未能抑制人甲状腺培养细胞中TSH反应性cAMP的积累。
