Dawson G J, Yates V J, Chang P W, Oprandy J J
Nature. 1982 Aug 5;298(5874):580-2. doi: 10.1038/298580a0.
The adeno-associated viruses (AAV) are defective parvoviruses which produce infective progeny only in cells co-infected with a 'helper' adenovirus (Ad). Both human and simian AAV have been recovered from human and simian primary cell cultures following their inoculation with 'AAV-free' Ad. Whereas some studies have suggested that AAV exists in a latent state in these cells, others have indicated that the AAV genome is capable of establishing and maintaining a latent state in defined laboratory conditions which mimic the situation proposed for the 'latent' AAV recovered from human and simian tissues. Here, avian adeno-associated virus (AAAV) was consistently recovered from limiting dilutions of purified and unpurified avian Ad stocks propagated in embryonating chicken eggs derived from two independently raised flocks of White Leghorn (WL) chickens but not when these Ad stocks were propagated in duck cells. These observations suggest that AAAV is a latent endogenous virus of at least some flocks of WL chickens.
腺相关病毒（AAV）是缺陷型细小病毒，只有在与“辅助”腺病毒（Ad）共同感染的细胞中才能产生感染性后代。在用人源和猴源的“无AAV”腺病毒接种人源和猴源原代细胞培养物后，已从中分离出人类和猿猴AAV。尽管一些研究表明AAV在这些细胞中处于潜伏状态，但其他研究表明，AAV基因组能够在特定的实验室条件下建立并维持潜伏状态，这些条件模拟了从人类和猿猴组织中分离出的“潜伏”AAV的情况。在这里，从在来自两个独立饲养的白来航（WL）鸡群的鸡胚中繁殖的纯化和未纯化禽腺病毒原液的有限稀释液中始终能分离出禽腺相关病毒（AAAV），但当这些腺病毒原液在鸭细胞中繁殖时则不能。这些观察结果表明，AAAV是至少一些WL鸡群的潜伏性内源病毒。
