Eaton C L, Pierrepoint C G
Prostate. 1982;3(3):277-90. doi: 10.1002/pros.2990030309.
Cell cultures displaying exclusively epithelial or fibroblastoid morphology have been isolated by spillage and collagenase digest techniques, respectively. Primary cultures of both cell types have been readily subcultured. The use of a type I collagen substrate has been shown to be essential to the growth of normal prostatic epithelium in monolayer cultures. The ability to generate replicate subcultures of both cell types has allowed the quantitative characterization of the mitogenicity of fetal bovine serum and insulin in early subcultures. The control of culture conditions has permitted uniform cell population growth in early subculture with regular population doubling times in log phase of growth. Epithelial cultures have been shown to display many ultrastructural characteristics common to the normal epithelium of the canine prostate.
分别通过溢出法和胶原酶消化技术分离出了仅呈现上皮形态或成纤维细胞样形态的细胞培养物。这两种细胞类型的原代培养物都很容易进行传代培养。已证明在单层培养中，I型胶原底物对正常前列腺上皮细胞的生长至关重要。能够对这两种细胞类型进行重复传代培养，从而可以对早期传代培养物中胎牛血清和胰岛素的促有丝分裂活性进行定量表征。对培养条件的控制使得早期传代培养中的细胞群体能够均匀生长，在对数生长期具有规律的群体倍增时间。上皮细胞培养物已显示出具有犬前列腺正常上皮细胞共有的许多超微结构特征。
