Afanas'eva T P, Filippovich S Iu, Kritskiĭ M S
Prikl Biokhim Mikrobiol. 1982 May-Jun;18(3):376-82.
The specific activity and molecular forms of NAD-kinase during ontogenesis of Neurospora crassa were investigated. The specific activity of the enzyme increased drastically at critical stages of fungal development, i.e. during conidia germination and during transition from the logarithmic to stationary growth stage, reaching 85 nmole NADP/hr/mg protein. By polyacrylamide gel electrophoresis four forms of NAD-kinase were revealed that had the following molecular masses: I-338,000, II-306,000, III-229,000, and IV-203,000. The vegetative mycelium contained predominantly form III, and conidia showed a high content of high-molecular-weight forms I and II.
对粗糙脉孢菌个体发育过程中NAD激酶的比活性和分子形式进行了研究。该酶的比活性在真菌发育的关键阶段急剧增加，即在分生孢子萌发期间以及从对数生长期向稳定生长期转变期间，达到85纳摩尔NADP/小时/毫克蛋白质。通过聚丙烯酰胺凝胶电泳揭示了四种NAD激酶形式，其分子量如下：I-338,000、II-306,000、III-229,000和IV-203,000。营养菌丝体主要含有III型，分生孢子显示出高分子量的I型和II型的高含量。
