Chen S, Lao W, Ni Z, Shi L
Sci Sin B. 1982 Mar;25(3):261-7.
Gene libraries of Rana nigromaculata and Mus musculus (C57J6) have been constructed by the cosmid system developed by Collins using BamHl and Pstl as cloning restriction enzymes. A simplified formula, which is a modification of Clarke and Carbon derived from approximation, was used for calculating the number of colonies or recombinants necessary for the construction of a gene library. Pro+ and leu+ complementary to the recipient cells (HB101) with the genetic constitution of pro- and leu- have been selected from the BamH1 gene libraries of both Rana nigromaculata and the mouse in the present work.
利用柯林斯开发的黏粒系统，以BamHl和Pstl作为克隆限制酶，构建了黑斑蛙和小家鼠（C57J6）的基因文库。采用了一个简化公式来计算构建基因文库所需的菌落或重组体数量，该公式是对克拉克和卡尔文公式进行近似修正得到的。在本研究中，从黑斑蛙和小鼠的BamH1基因文库中筛选出了与遗传组成为pro-和leu-的受体细胞（HB101）互补的Pro+和Leu+。
