Sargeaunt P G, Williams J E, Bhojnani R, Campos J E, Gomez A
Trans R Soc Trop Med Hyg. 1982;76(2):208-10. doi: 10.1016/0035-9203(82)90276-0.
Stocks of intestinal amoebae grown in monoxenic culture, were compared against each other and against those previously reported from Mexico City. These were isolated from subjects in San Cristobal de las Casas, Chiapas (rural area) and hospital patients in Merida, Yucatan (urban area). Electrophoretic patterns of the four enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM), L-malate: NADP+ oxido-reductase (oxalacetate-decarboxylating) (ME) and hexokinase (HK) demonstrated the presence of five groups (zymodemes) of Entamoeba histolytica already described from Mexico City, together with two new zymodemes, one of which gave a recognizable pathogenic pattern, whilst the other gave a contradictory pattern. Zymodemes of Entamoeba coli, Entamoeba hartmanni, Iodamoeba buetschlii and Dientamoeba fragilis, previously described were also isolated. One new zymodeme of D. fragilis was demonstrated.
将在单菌培养中生长的肠道阿米巴菌株相互比较，并与之前从墨西哥城报道的菌株进行比较。这些菌株分别从恰帕斯州圣克里斯托瓦尔·德拉斯卡萨斯的受试者（农村地区）和尤卡坦州梅里达的医院患者（城市地区）中分离得到。四种酶，即葡萄糖磷酸异构酶（GPI）、磷酸葡萄糖变位酶（PGM）、L-苹果酸：NADP+氧化还原酶（草酰乙酸脱羧）（ME）和己糖激酶（HK）的电泳图谱显示，存在已从墨西哥城描述的五组溶组织内阿米巴（酶带型），以及两个新的酶带型，其中一个呈现出可识别的致病模式，而另一个呈现出矛盾的模式。之前描述的结肠内阿米巴、哈氏内阿米巴、布氏嗜碘阿米巴和脆弱双核阿米巴的酶带型也被分离出来。还发现了一种新的脆弱双核阿米巴酶带型。
