[宋内志贺菌中发现的pKMR质粒的特征]

[Characteristics of the pKMR plasmids found in Shigella flexneri].

作者信息

Korotiaev A I, Sidorov I A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1982 May(5):41-3.

Abstract

The clinical isolate of Sh. flexneri 1b, resistant to 5 antibiotics and sulfonamides, has been studied by the method of conjugation and found to have a group of transfer-suppressed pKMR-plasmids: pKMR 204-1 (Ap Sm Tc Cm Km Su), pKMR 204-2 (Sm Km Su), pKMR 204-5 (Km Su) and pKMR 204-7 (Sm Tc Cm Km Su), whose molecular weight was 99, 71.2, 73.8 and 59.5 Md respectively. The treatment of the plasmids with restriction endonuclease BamHI has revealed that plasmids pKMR 204-2 and pKMR 204-5 are definitely related to plasmid KMR 204-1, being its deletion mutants. At the same time plasmids pKMR 204-1 and pKMR 204-7 differ in their sensitivity to endonuclease BamHI and stably coexist within the same cell, thus seeming to belong to different compatibility groups.

摘要

对一株对5种抗生素和磺胺类药物耐药的弗氏志贺菌1b临床分离株进行了接合试验研究，发现它含有一组转移受抑制的pKMR质粒：pKMR 204 - 1（Ap Sm Tc Cm Km Su）、pKMR 204 - 2（Sm Km Su）、pKMR 204 - 5（Km Su）和pKMR 204 - 7（Sm Tc Cm Km Su），其分子量分别为99、71.2、73.8和59.5兆道尔顿。用限制性内切酶BamHI处理这些质粒后发现，质粒pKMR 204 - 2和pKMR 204 - 5肯定与质粒KMR 204 - 1相关，是其缺失突变体。同时，质粒pKMR 204 - 1和pKMR 204 - 7对核酸内切酶BamHI的敏感性不同，并能在同一细胞内稳定共存，因此似乎属于不同的相容性群。