Polatnick J, Wool S H
Arch Virol. 1982;71(3):207-15. doi: 10.1007/BF01314872.
Viral RNA synthesis in foot-and-mouth disease infected bovine kidney cell cultures was associated throughout the infectious period with newly formed smooth membranous vacuoles. Membrane formation was measured by choline uptake. The site of RNA synthesis was determined by electron microscopic examination of autoradiograms of incorporated [3H] uridine. Both membrane formation and RNA synthesis became significant at 2.5 hours postinfection, but membrane formation increased steadily to 4.5 hours while RNA synthesis peaked at 3.5 hours. Percent density distributions of developed silver grains on autoradiograms showed that almost all RNA synthesis was concentrated on the smooth vacuoles of infected cells. Histogram analysis of grain density distributions established that the sites of RNA synthesis was the vacuolar membrane. The newly formed smooth membrane-bound vacuoles were not seen to coalesce into the large vacuolated areas typical of poliovirus cytopathogenicity.
在口蹄疫感染的牛肾细胞培养物中，病毒RNA合成在整个感染期都与新形成的光滑膜性空泡有关。通过胆碱摄取来测量膜形成。通过对掺入的[3H]尿苷的放射自显影片进行电子显微镜检查来确定RNA合成的位点。膜形成和RNA合成在感染后2.5小时均变得显著，但膜形成持续增加至4.5小时，而RNA合成在3.5小时达到峰值。放射自显影片上显影银粒的百分比密度分布表明，几乎所有RNA合成都集中在感染细胞的光滑空泡上。银粒密度分布的直方图分析确定RNA合成的位点是液泡膜。未观察到新形成的光滑膜结合空泡聚合并形成脊髓灰质炎病毒细胞致病性典型的大空泡化区域。
