Polatnick J, Wool S H
Comp Immunol Microbiol Infect Dis. 1983;6(3):265-72. doi: 10.1016/0147-9571(83)90019-x.
The localization of foot-and-mouth disease viral-induced RNA polymerase has been determined in situ and in partially fractionated cell components by using polymerase antisera tagged with either peroxidase or ferritin. Electron microscopic examination revealed the polymerase to be heavily concentrated on membranes of the smooth membranous vacuoles (SMV) which are newly formed during infection and which were previously shown to be the site where newly synthesized viral RNA appeared. Polymerase antigen was also seen to be associated with the endoplasmic reticulum (ER), the assumed site of original synthesis, and to a lesser extent with mitochondria and the Golgi apparatus. There was no significant polymerase attachment to nuclear and plasma membranes.
利用用过氧化物酶或铁蛋白标记的聚合酶抗血清，对口蹄疫病毒诱导的RNA聚合酶进行了原位定位及在部分分级分离的细胞组分中的定位。电子显微镜检查显示，聚合酶大量集中在感染期间新形成的光滑膜泡（SMV）的膜上，此前已表明该部位是新合成的病毒RNA出现的位点。还发现聚合酶抗原与内质网（ER）（推测为原始合成位点）相关，与线粒体和高尔基体的相关性较小。在核膜和质膜上没有明显的聚合酶附着。
