针对纯化的兔滑膜成纤维细胞胶原酶制备单特异性抗体。
Preparation of a monospecific antibody to purified rabbit synovial fibroblast collagenase.
作者信息
Vater C A, Hahn J L, Harris E D
出版信息
Coll Relat Res. 1981 Nov;1(6):527-42. doi: 10.1016/s0174-173x(81)80034-9.
PMID:6286237
Abstract
Collagenase produced by monolayer cultures of rabbit synovial fibroblasts was purified from serum-free culture medium. Antiserum to the pure preparation was raised in sheep. The IgG fraction has been isolated and characterized and shown to be specific for collagenase. The antibody is capable of precipitating and inhibiting the activity of both latent and activated rabbit synovial fibroblast collagenase. Cross-reactivity with human antigen from rheumatoid synovial cell culture medium is described.
摘要
从兔滑膜成纤维细胞单层培养物产生的胶原酶是从无血清培养基中纯化得到的。用该纯制剂在绵羊体内制备了抗血清。已分离并鉴定了IgG组分，结果表明它对胶原酶具有特异性。该抗体能够沉淀并抑制潜伏型和活化型兔滑膜成纤维细胞胶原酶的活性。本文还描述了其与类风湿滑膜细胞培养基中的人抗原的交叉反应性。
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