Mogensen S C
Immunobiology. 1982;162(1):28-38. doi: 10.1016/S0171-2985(11)80014-8.
Mice inoculated intraperitoneally or intravenously with herpes simplex virus type 2 (HSV-2) develop a focal necrotizing hepatitis. The livers show expanding foci of necrosis and increasing virus content during the first days of the infection with maximal titers achieved on day 3. The clearance of virus from the organ is manifest from day 4 onward with the most dramatic fall in virus content occurring between days 4 and 5. The development of immunity during the course of infection was assessed by adoptive transfer experiments and by measuring macrophage migration inhibition factor (MIF) production of spleen cells in an indirect agarose microdroplet assay. Antiviral activity of adoptively transferred spleen cells was demonstrable from day 4 of the infection when 50 X 10(6) spleen cells were transferred into recipient mice infected 24 h previously. MIF production in spleen cell cultures stimulated with antigen was found to be specific in that activity was only detected in cultures derived from immune mice and stimulated with the virus antigen. The response was found to be antigen-dose and cell-number dependent. Significant MIF production was demonstrable in spleen cell cultures derived from mice 3 days after the infection, i.e. concomitant with the initiation of recovery and before antiviral activity can be detected in transfer experiments. It is suggested that a delayed type hypersensitivity reaction with lymphokine production leading to recruitment of macrophages and their retention and activation in the foci of infection may be a major factor in the recovery from the infection.
经腹腔内或静脉内接种2型单纯疱疹病毒（HSV-2）的小鼠会发生局灶性坏死性肝炎。在感染的最初几天，肝脏显示出坏死灶不断扩大且病毒含量增加，在第3天达到最高滴度。从第4天起，病毒开始从器官中清除，病毒含量在第4天至第5天之间下降最为显著。通过过继转移实验以及在间接琼脂糖微滴试验中测量脾细胞产生巨噬细胞移动抑制因子（MIF）来评估感染过程中免疫的发展。当将50×10⁶个脾细胞转移到24小时前已感染的受体小鼠中时，从感染第4天起就可证明过继转移的脾细胞具有抗病毒活性。发现用抗原刺激的脾细胞培养物中MIF的产生具有特异性，因为仅在来自免疫小鼠且用病毒抗原刺激的培养物中检测到活性。发现该反应取决于抗原剂量和细胞数量。在感染后3天的小鼠脾细胞培养物中可证明有显著的MIF产生，即在恢复开始时且在转移实验中检测到抗病毒活性之前。有人提出，伴有淋巴因子产生的迟发型超敏反应导致巨噬细胞募集并在感染灶中滞留和激活，这可能是从感染中恢复的主要因素。
