Hamer D H, Walling M
J Mol Appl Genet. 1982;1(4):273-88.
Cadmium and other heavy metals induce metallothionein synthesis in mammalian cells. We have cloned a mouse metallothionein-I gene from a cadmium-resistant line of Ltk- cells and inserted it into viral and plasmid SV40 vectors. These recombinant molecules were introduced into cultured monkey cells and the expression of the foreign metallothionein gene was analyzed, at the RNA level, in the presence and absence of cadmium. The results show that the cloned gene retains its cadmium inducibility and that the regulation of this gene occurs primarily at the transcriptional level.
镉及其他重金属可诱导哺乳动物细胞合成金属硫蛋白。我们从抗镉的Ltk-细胞系中克隆了小鼠金属硫蛋白-I基因，并将其插入病毒和质粒SV40载体中。将这些重组分子导入培养的猴细胞中，并在有镉和无镉的情况下，从RNA水平分析外源金属硫蛋白基因的表达。结果表明，克隆的基因保留了其对镉的诱导性，并且该基因的调控主要发生在转录水平。
