维生素C缺乏的豚鼠肝脏微粒体中细胞色素P-450的NADPH依赖性还原：苄非他明的作用
NADPH-dependent reduction of cytochrome P-450 in liver microsomes from vitamin C-deficient guinea pigs: effect of benzphetamine.
作者信息
Rikans L E
出版信息
J Nutr. 1982 Sep;112(9):1796-800. doi: 10.1093/jn/112.9.1796.
The effect of dietary vitamin C on the reduction of cytochrome c and cytochrome P-450 by NADPH-cytochrome P-450 reductase was determined in guinea pig liver microsomes. Acute vitamin C deficiency decreased hepatic microsomal cytochrome P-450 content 21% and the rate of cytochrome P-450 reduction 66% without affecting cytochrome c reduction. However, the vitamin status of the animals did not affect the enhancement in cytochrome P-450 reduction produced by the addition of a type I substrate to the reaction mixture. The results suggest that vitamin C deficiency selectivity affects the transfer of electron from NADPH to cytochrome P-450 and that this effect does not result from a change in the spin state of cytochrome P-450.
在豚鼠肝微粒体中测定了膳食维生素C对NADPH-细胞色素P-450还原酶还原细胞色素c和细胞色素P-450的影响。急性维生素C缺乏使肝微粒体细胞色素P-450含量降低21%，细胞色素P-450还原速率降低66%，而不影响细胞色素c的还原。然而，动物的维生素状态并不影响向反应混合物中添加I型底物所产生的细胞色素P-450还原增强。结果表明，维生素C缺乏选择性地影响电子从NADPH向细胞色素P-450的转移，且这种影响并非由细胞色素P-450自旋状态的改变所致。
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