Shephard E A, Jansing R L, Phillips I R, Stein J L, Stein G S
Anticancer Res. 1981;1(2):89-92.
The representation of mRNA sequences in normal and SV40-transformed WI-38 human diploid fibroblasts was examined. Variations in the hybridization of polysomal RNAs to 3H-labeled DNAs complementary to poly A+ mRNA of normal and SV40-transformed cells suggest differences in the mRNA populations. a comparison of the representation of histone RNA sequences of normal and SV40-transformed human diploid cells did not reveal significant differences.
对正常和经SV40转化的WI-38人二倍体成纤维细胞中mRNA序列的表现进行了检测。多核糖体RNA与正常细胞和经SV40转化细胞的聚腺苷酸加尾mRNA的3H标记互补DNA杂交的差异表明mRNA群体存在差异。对正常和经SV40转化的人二倍体细胞组蛋白RNA序列表现的比较未发现显著差异。