Moronne M M, Cohen J A
Biochim Biophys Acta. 1982 Jun 28;688(3):793-7. doi: 10.1016/0005-2736(82)90293-0.
The voltage-sensitive channel-former monazomycin is used as a conductance probe to monitor changes in the trans electrostatic surface potentials of negatively-charged planar phospholipid bilayers. Cis-to-trans electroneutral fluxes of divalent cations mediated by ionophores A23187 and X537A are sensed via the effect of transported divalent cations on the trans surface potentials. Quantitative determinations of neutral Ca2+ and Mg2+ fluxes are made and related to ionophore function.
电压敏感通道形成剂莫那霉素被用作电导探针，以监测带负电荷的平面磷脂双分子层跨膜静电表面电位的变化。通过离子载体A23187和X537A介导的二价阳离子的顺式到反式电中性通量，是通过被转运的二价阳离子对反式表面电位的影响来检测的。对中性Ca2+和Mg2+通量进行了定量测定，并将其与离子载体功能相关联。
