[蛋白质硫醇与不同大鼠肝癌倍增时间之间的相关性]
[Correlation between protein thiols and the doubling time of various rat hepatomas].
作者信息
Carow H M, Kharrazi H, Khoschsorur G, Schauenstein E, Schaur R J, Tillian H M, Morris H P
出版信息
J Cancer Res Clin Oncol. 1982;103(3):281-6. doi: 10.1007/BF00409703.
The SH content of the soluble proteins (nanomol./mg protein) from five transplantable rat hepatomas and from the DENA-hepatoma were determined with dithionitrobenzoate (Ellman reagent). Both the total number of thiols as well as the number of SH groups that can be blocked by hydroxypentenal (HPE) increase with increasing growth rate of the tumors. In comparison with the protein thiol content of the slowest growing DENA-hepatoma (doubling time 100 days), the total protein thiols of the fastest growing Yoshida hepatoma (doubling time 2,5 days) increase by 100% and the HPE-sensitive protein thiols by 350%. The total protein thiols are significantly correlated with the growth rate (probability of error 5%), the HPE-sensitive thiols are correlated with high significance (probability of error less than 1%). These results are in accordance with the "Molecular Correlation Concept" of G. Weber and might possibly be understood as a consequence of reprogramming of gene expressions.
用二硫代硝基苯甲酸（埃尔曼试剂）测定了五种可移植大鼠肝癌以及二乙基亚硝胺诱导肝癌的可溶性蛋白质中的巯基含量（纳摩尔/毫克蛋白质）。随着肿瘤生长速度的加快，总巯基数量以及可被羟基戊二醛（HPE）阻断的巯基数量均增加。与生长最慢的二乙基亚硝胺诱导肝癌（倍增时间为100天）的蛋白质巯基含量相比，生长最快的吉田肝癌（倍增时间为2.5天）的总蛋白质巯基增加了100%，对HPE敏感的蛋白质巯基增加了350%。总蛋白质巯基与生长速度显著相关（误差概率为5%），对HPE敏感的巯基与生长速度高度显著相关（误差概率小于1%）。这些结果与G. 韦伯的“分子相关概念”一致，并且可能被理解为基因表达重编程的结果。
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