肝癌细胞系中存在非染色体的乙肝病毒表面（HBsAg）和核心抗原（HBcAg）特异性DNA序列的证据。

Evidence for non-chromosomal hepatitis B virus surface (HBsAg)- and core antigen (HBcAg)-specific DNA sequences in a hepatoma cell line.

作者信息

Marquardt O, Zaslavsky V, Hofschneider P H

出版信息

J Gen Virol. 1982 Jul;61 (Pt l):105-9. doi: 10.1099/0022-1317-61-1-105.

DOI:10.1099/0022-1317-61-1-105

Abstract

As demonstrated previously, a "beta particle" fraction isolated from the cytoplasm of PLC/PRF/5 cells contains hepatitis B virus (HBV)-specific DNA. Here, further evidence is provided that the specificity of the DNA for HBV is represented at least by sequences coding for the surface and core antigen (HBsAg and HBcAg). This was shown by two different hybridization techniques. One of them, the technique of Southern, distinguished these hybrid molecules formed from those containing HBV DNA integrated into chromosomes. The HBV-specific beta particle DNA forms two distinct bands separate from the high molecular weight cellular DNA.

摘要

如先前所示，从PLC/PRF/5细胞胞质中分离出的“β粒子”组分含有乙型肝炎病毒（HBV）特异性DNA。在此，进一步证明了该DNA对HBV的特异性至少由编码表面抗原和核心抗原（HBsAg和HBcAg）的序列所体现。这通过两种不同的杂交技术得以证实。其中之一是Southern技术，它可区分由整合到染色体中的HBV DNA形成的杂交分子与这些杂交分子。HBV特异性β粒子DNA形成两条与高分子量细胞DNA分开的独特条带。

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