Fenwick M L, Clark J
J Gen Virol. 1982 Jul;61 (Pt l):121-5. doi: 10.1099/0022-1317-61-1-121.
A mutant of herpes simplex virus type 1 [HSV-1(HFEM)], tsB7, appears to have two temperature-sensitive functions. One is required during the first hour of infecting a cell (suggesting that it is performed by a virion protein) and the other is the non-essential function of "early shut-off" of cellular protein synthesis, which is mediated by a virion protein. The latter function remained temperature-sensitive in a revertant virus (RC2) grown at the non-permissive temperature (39 degrees C). However, under these conditions RC2 did cause inhibition of host synthesis, showing that "delayed shut-off", requiring virus protein synthesis, can occur independently of early shut-off, which is mediated by a virion protein. Early shut-off by u.v.-irradiated tsB7 was reversed when the temperature was raised, whereas delayed shut-off by intact tsB7 was not. Of two wild-type strains of virus examined, HSV-1(F) also exhibited temperature-sensitive early shut-off, but HSV-2(G) did not.
单纯疱疹病毒1型[HSV-1(HFEM)]的一个突变体tsB7似乎具有两种温度敏感功能。一种功能在感染细胞的最初一小时内是必需的（这表明它是由一种病毒体蛋白执行的），另一种功能是细胞蛋白质合成“早期关闭”的非必需功能，该功能由一种病毒体蛋白介导。在非允许温度（39摄氏度）下生长的回复病毒（RC2）中，后一种功能仍然对温度敏感。然而，在这些条件下，RC2确实会导致宿主合成受到抑制，这表明需要病毒蛋白质合成的“延迟关闭”可以独立于由病毒体蛋白介导的早期关闭而发生。当温度升高时，紫外线照射的tsB7引起的早期关闭会被逆转，而完整的tsB7引起的延迟关闭则不会。在所检测的两种野生型病毒株中，HSV-1(F)也表现出温度敏感的早期关闭，但HSV-2(G)则没有。
