Zendegui J G, Klein T W
J Reticuloendothel Soc. 1982 Jun;31(6):455-67.
Cyclic nucleotides have been implicated in the normal function of macrophages, but the nature of the cyclic nucleotide catabolizing enzyme phosphodiesterase (PDE) and its role in regulation of macrophage function has not been previously reported. Cyclic adenosine monophosphate PDE activity was studied in mouse peritoneal macrophages and found to be similar to that reported in other tissues with regard to stability and divalent cation requirement. Exudate macrophages induced by injection of either endotoxin or proteose peptone broth were found to have reduced PDE activity and enhanced EAC phagocytic capacity and acid phosphatase (AP) activity. When resident peritoneal macrophages were cultured in vitro; AP activity increased over 24 hr and remained elevated for 96 hr. PDE activity declined steadily over 96 hr. This steady decline in PDE activity observed during culture was not altered by inclusion of endotoxin. These studies suggest that PDE activity is reduced in macrophages with maximally enhanced function but does not appear to be significantly altered during early phases of functional change in macrophages.
环核苷酸与巨噬细胞的正常功能有关，但环核苷酸分解代谢酶磷酸二酯酶（PDE）的性质及其在巨噬细胞功能调节中的作用此前尚未见报道。对小鼠腹腔巨噬细胞中的环磷酸腺苷磷酸二酯酶活性进行了研究，发现其在稳定性和二价阳离子需求方面与其他组织中报道的情况相似。发现注射内毒素或蛋白胨肉汤诱导的渗出液巨噬细胞具有降低的磷酸二酯酶活性以及增强的EAC吞噬能力和酸性磷酸酶（AP）活性。当驻留腹腔巨噬细胞在体外培养时，AP活性在24小时内增加并在96小时内保持升高。磷酸二酯酶活性在96小时内稳步下降。在培养过程中观察到的磷酸二酯酶活性的这种稳步下降并未因加入内毒素而改变。这些研究表明，在功能最大程度增强的巨噬细胞中磷酸二酯酶活性降低，但在巨噬细胞功能变化的早期阶段似乎没有明显改变。
