Twardzik D R, Sherwin S A, Ranchalis J, Todaro G J
J Natl Cancer Inst. 1982 Oct;69(4):793-8.
Transforming growth factor (TGF) activities could be detected in the urine of normal, pregnant, and tumor-bearing humans. These acid- and heat-stable polypeptides competed for binding to epidermal growth factor (EGF) membrane receptors and promoted the anchorage-independent growth of nontransformed rodent cells. They differed from human EGF in their apparent molecular weights and soft-agar growth-stimulating activity. The urine from pregnant females contained TGF activities with apparent molecular weight(s) (relative) (Mr) of 10,000 ad 17,000--20,000. In the case of a lung cancer patient, an additional major activity of approximately 30,000--35,000 Mr was found. All urine specimens examined also contained a "common" 8,000-Mr soft-agar growth-stimulating activity, which competed for binding to EGF membrane receptors and which was chromatographically separable from EGF (urogastrone). Thus urine may provide a convenient and readily available source for the biochemical characterization of these TGF-like activities, some of which may be clinically useful biologic markers for certain types of cancer.
在正常、怀孕和患肿瘤的人体尿液中均可检测到转化生长因子（TGF）活性。这些耐酸且耐热的多肽可竞争结合表皮生长因子（EGF）膜受体，并促进未转化的啮齿动物细胞的非贴壁依赖性生长。它们在表观分子量和软琼脂生长刺激活性方面与人类EGF不同。怀孕女性尿液中的TGF活性表观分子量（相对）（Mr）为10,000以及17,000 - 20,000。在一名肺癌患者的尿液中，还发现了一种额外的主要活性物质，其Mr约为30,000 - 35,000。所有检测的尿液标本中还含有一种“常见的”8,000-Mr软琼脂生长刺激活性物质，它可竞争结合EGF膜受体，并且在色谱上可与EGF（尿抑胃素）分离。因此，尿液可能为这些TGF样活性物质的生化特性研究提供一个方便且容易获取的来源，其中一些可能是某些类型癌症的具有临床意义的生物学标志物。
