Cooper H S
Lab Invest. 1982 Oct;47(4):383-90.
Peanut lectin is known to bind to B-D-Gal-(1 leads to 3)-D-GalNac which provides antigenic determination for the T (TAg) blood group antigen. We examined 33 rectosigmoid carcinomas and 15 corresponding controls for their ability to express peanut lectin-binding sites. In controls one could localize TAg to the supranuclear portion of the cell, however, in cancers one noticed a cytostructural relocalization of TAg with the following two major patterns: localization to the region of the glycocalyx and localization intracytoplasmically in the apical portion of the cell. These two patterns were associated with glandular differentiation. Less frequently noted or in association with the above was a mucin glob-like pattern and/or a fine diffuse intracytoplasmic pattern associated with solid, nonglandular areas. The more poorly differentiated cancers less frequently expressed peanut lectin-binding sites. Benign (nontransitional zone) epithelium in those patients whose tumor expressed TAg was negative for peanut lectin-binding sites in 66 per cent of the cases. Reduced tumoral glycosyltransferases may explain this increased synthesis of TAg in cancers as compared with controls, if one considers TAg to be an incomplete glycoprotein of the MN blood group system.
已知花生凝集素可与β-D-半乳糖-（1→3）-D-氨基半乳糖结合，后者为T（TAg）血型抗原提供抗原决定簇。我们检测了33例直肠乙状结肠癌及15例相应对照表达花生凝集素结合位点的能力。在对照中，可将TAg定位于细胞的核上部分，然而，在癌组织中，可观察到TAg的细胞结构重新定位，主要有以下两种模式：定位于糖萼区域及定位于细胞顶端部分的胞质内。这两种模式与腺管分化有关。较少见的或与上述情况相关的是与实性、非腺管区域相关的粘蛋白球状模式和/或细微的弥漫性胞质内模式。分化较差的癌组织表达花生凝集素结合位点的频率较低。在肿瘤表达TAg的患者中，66%的病例其良性（非移行区）上皮花生凝集素结合位点呈阴性。如果将TAg视为MN血型系统的一种不完全糖蛋白，那么肿瘤糖基转移酶减少可能解释了与对照相比癌组织中TAg合成增加的现象。
