通过使用“牛奶孕酮检测法”对牛进行生育控制。

Fertility control in cattle by use of the "milk progesterone test".

作者信息

van de Wiel D F, van Eldik J, Koops W, Postma A, Oldenbroek J K

出版信息

Tijdschr Diergeneeskd. 1978 Jan 15;103(2):91-103.

Abstract

The development and validation of a radioimmunoassay for progesterone in cow's milk is described. Control of the temperature during storage of the milk and also during the sample incubation procedure appeared to be a critical requirement with respect to reduction of nonspecific binding of tritiated progesterone. Ranges of milk progesterone levels were established during pregnancy as well as during the luteal and the follicular phase of the oestrus and of pregnancy in three trials on two experimental farms. The mean accuracy with respect to the diagnosis "pregnant", "non-pregnant" or "not in oestrus" were 84.0, 97.6, and 100%, respectively. By leaving out of account all animals with high progesterone values (greater than 10 ng. ml(-1)) on day 1 (= day of insemination) the accuracy of the diagnosis "pregnant" was improved to 87.1%.

摘要

本文描述了一种用于测定牛奶中孕酮的放射免疫分析方法的开发与验证。在牛奶储存以及样品孵育过程中控制温度，对于减少氚标记孕酮的非特异性结合似乎至关重要。在两个实验农场进行的三项试验中，确定了妊娠期间以及发情期和妊娠期黄体期及卵泡期的牛奶孕酮水平范围。对于“怀孕”、“未怀孕”或“未发情”诊断的平均准确率分别为84.0%、97.6%和100%。若不考虑授精当天（第1天）孕酮值高（大于10 ng·ml⁻¹）的所有动物，“怀孕”诊断的准确率提高到了87.1%。