Shatalin K Iu, Romanova Iu M, Zakharenko V I
Zh Mikrobiol Epidemiol Immunobiol. 1982 Jul(7):79-82.
The insertion sites of elements Tn9 and Tn601 which determine chloramphenicol and kanamycin resistance have been detected restriction analysis. The functioning of transposons i.e. their stability or instability, has been found to influence the specificity of their insertions into the genome of lambda att80 bacteriophage. During transposition from stable integration sites both transposons are inserted into the regions of the lambda att80 bacteriophage genome, definite for each transposon. However, during transposition from the site of unstable integration both determinants of drug resistance are inserted into different regions of the phage genome.
通过限制性分析已检测到决定氯霉素和卡那霉素抗性的Tn9和Tn601元件的插入位点。已发现转座子的功能，即它们的稳定性或不稳定性，会影响它们插入λatt80噬菌体基因组的特异性。在从稳定整合位点进行转座的过程中，两个转座子都插入到λatt80噬菌体基因组的特定区域，每个转座子的插入区域都是确定的。然而，在从不稳定整合位点进行转座的过程中，两种耐药性决定因素都插入到噬菌体基因组的不同区域。
