Defendini R, Zimmerman E A, Weare J A, Alhenc-Gelas F, Edros E G
Adv Biochem Psychopharmacol. 1982;33:271-80.
Homogeneous human CE hydrolyzed Met5-enkephalin fastest of the biologically active substrates tested. The inactivation of Met5-enkephalin was inhibited by antiserum to Ce and by specific inhibitor of CE (MK 421; I50 = 8 x 10(-9)M). Direct radioimmunoassay of CE extracted from basal ganglia indicated immunological identity of lung, kidney, and brain CE. Immunocytochemically, CE was demonstrated in the choroid plexus, where the enzyme appears to be bound, as in kidney and gut, to the surface of the epithelial cell bathed by luminal fluid. CE immunoreactivity in brain parenchyma was demonstrated in neuronal elements in the hippocampus, hypothalamus, neocortex, globus pallidus, and substantia nigra.
在测试的生物活性底物中，纯合的人羧肽酶E（CE）对甲硫氨酸脑啡肽五肽（Met5-脑啡肽）的水解速度最快。抗CE血清和CE的特异性抑制剂（MK 421；半数抑制浓度=8×10⁻⁹M）可抑制Met5-脑啡肽的失活。对从基底神经节提取的CE进行直接放射免疫测定，结果表明肺、肾和脑CE具有免疫同一性。免疫细胞化学显示，脉络丛中有CE，与肾和肠道一样，该酶似乎与被管腔液浸泡的上皮细胞表面结合。脑实质中的CE免疫反应性在海马、下丘脑、新皮层、苍白球和黑质的神经元成分中得到证实。
