Alhenc-Gelas F, Weare J A, Johnson R L, Erdös E G
J Lab Clin Med. 1983 Jan;101(1):83-96.
CE was purified from human lung, and antisera were raised in rabbits. Antisera inhibited the activity of the purified enzyme from lung and kidney and the plasma CE of normal persons and sarcoid patients. With antisera at a titer of 1:100,000, a sensitive, direct RIA was developed. CE purified from lung or kidney and CE present in normal and in sarcoid plasma gave parallel logit-log displacement lines, suggesting immunological identity. The level of CE in normal human plasma was 400 +/- 131 ng/ml. In untreated sarcoid patients, the enzyme level and activity increased in parallel. There was a negative correlation (r = -0.81) between enzyme level and diffusing capacity of the lung for CO in sarcoid patients. Synthetic inhibitors such as captopril or MK 421 did not interfere with the RIA, permitting enzyme levels to be monitored in patients undergoing acute inhibitor therapy. During administration of MK 421, CE activity was negligible and plasma levels of CE did not change. In contrast, renin activity increased eightfold during the inhibitor therapy.
从人肺中纯化出CE，并在兔体内制备抗血清。抗血清抑制了来自肺和肾的纯化酶以及正常人和结节病患者血浆CE的活性。用效价为1:100,000的抗血清开发了一种灵敏的直接放射免疫分析法。从肺或肾中纯化的CE以及正常和结节病血浆中的CE给出了平行的对数-对数位移曲线，表明具有免疫同一性。正常人血浆中CE的水平为400±131 ng/ml。在未经治疗的结节病患者中，酶水平和活性平行升高。结节病患者的酶水平与肺对CO的弥散能力之间存在负相关（r = -0.81）。卡托普利或MK 421等合成抑制剂不干扰放射免疫分析法，从而能够在接受急性抑制剂治疗的患者中监测酶水平。在给予MK 421期间，CE活性可忽略不计，血浆CE水平未发生变化。相比之下，在抑制剂治疗期间肾素活性增加了八倍。
