Stefanovich L E, Eliseeva L S, Popova V S
Biokhimiia. 1982 Aug;47(8):1370-4.
The properties of serotonin-active sites were studied on peritoneal cells, blood leukocytes and synaptosomes of mice (CBA line). Treatment of cell suspensions with EDTA, ouabain, strophanthin, 2,4-dinitrophenol, dithiothreitol and trypsin demonstrated that serotonin binding by peritoneal cells and leukocytes depends on bivalent ions and K+, Na+-pump operation, requires energy and intact disulfide bonds and is determined by a protein structure. ATP and ADP were found to inhibit amine adsorption by peritoneal cells. These cells specifically bind ATP 10 times more intensively than leukocytes. The data obtained are suggestive of differences in the composition of serotonin-active structures of blood leukocytes, peritoneal cells and synaptosomes.
在小鼠（CBA品系）的腹膜细胞、血液白细胞和突触体上研究了5-羟色胺活性位点的特性。用乙二胺四乙酸（EDTA）、哇巴因、毒毛花苷、2,4-二硝基苯酚、二硫苏糖醇和胰蛋白酶处理细胞悬液表明，腹膜细胞和白细胞对5-羟色胺的结合依赖于二价离子和钾离子、钠离子泵的运转，需要能量和完整的二硫键，并且由蛋白质结构决定。发现三磷酸腺苷（ATP）和二磷酸腺苷（ADP）可抑制腹膜细胞对胺的吸附。这些细胞对ATP的特异性结合比白细胞强10倍。所获得的数据表明血液白细胞、腹膜细胞和突触体的5-羟色胺活性结构的组成存在差异。
