Gusterson B A, Warburton M J, Mitchell D, Ellison M, Neville A M, Rudland P S
Cancer Res. 1982 Nov;42(11):4763-70.
An immunocytochemical method for fixed and paraffin-embedded human breast biopsies is reported for the detection of myoepithelial and epithelial cells using antibodies to myosin and keratin, respectively, and of basement membranes using antibodies to laminin and type IV collagen. Using these markers, myoepithelial cells can be clearly distinguished in the normal breast and in the benign breast diseases sclerosing adenosis, epitheliosis, and fibroadenoma. In sclerosing adenosis, myoepithelial cells form a major cellular component. A stromally derived spindle cell is identified which stains with myosin but not with keratin antibodies (myofibroblast). These cells are seen in one-fifth of the fibroadenomas. Although cells staining with myosin antibodies are seen in the infiltrating component of all 18 carcinomas examined, elongated cells staining with both myosin and keratin antibodies (myoepithelial-like) are seen in only one infiltrating carcinoma where they are interposed at the stromal-epithelial junction of the infiltrating tumor cells. In contrast to the situation in benign breast diseases, mature myoepithelial cells form a very minor component of the majority of infiltrating ductal carcinomas. Basement membrane proteins, laminin, and type IV collagen are present in normal breast, benign breast disease, and grade I infiltrating ductal carcinomas but are absent in carcinomas of grades II and III.
本文报道了一种用于固定石蜡包埋的人乳腺活检组织的免疫细胞化学方法，该方法分别使用抗肌球蛋白和抗角蛋白抗体检测肌上皮细胞和上皮细胞，使用抗层粘连蛋白和IV型胶原抗体检测基底膜。利用这些标志物，在正常乳腺以及良性乳腺疾病（硬化性腺病、上皮增生和纤维腺瘤）中可清晰区分肌上皮细胞。在硬化性腺病中，肌上皮细胞构成主要细胞成分。识别出一种源自间质的梭形细胞，其用肌球蛋白抗体染色但不用角蛋白抗体染色（肌成纤维细胞）。在五分之一的纤维腺瘤中可见这些细胞。尽管在所检查的所有18例癌的浸润成分中均可见用肌球蛋白抗体染色的细胞，但仅在1例浸润性癌中可见同时用肌球蛋白和角蛋白抗体染色的细长细胞（肌上皮样细胞），它们位于浸润性肿瘤细胞的间质 - 上皮交界处。与良性乳腺疾病的情况相反，成熟的肌上皮细胞在大多数浸润性导管癌中仅占非常小的成分。基底膜蛋白、层粘连蛋白和IV型胶原存在于正常乳腺、良性乳腺疾病和I级浸润性导管癌中，但在II级和III级癌中不存在。
