East J M, Dutton G R
Dev Neurosci. 1982 Mar-Jun;5(2-3):117-24. doi: 10.1159/000112668.
[3H]-dihydroalprenolol was used to examine the development of beta-adrenoceptors in the cerebellum of weaver, reeler, staggerer, and jimpy neurologically mutant mice and their normal counterparts. In normal animals the greatest increase in [3H] binding occurred during the postnatal interval of 10-15 days, and maximum values were obtained at about 3 weeks. Binding was saturable with an apparent dissociation constant of 0.26 nM, and the affinity of [3]-dihydroalprenolol for its receptor did not change significantly during development. [3H]-dihydroalprenolol binding was significantly reduced in weaver, reeler, and staggerer (14-22% of control values) at 20 days, but not in the jimpy mutant. These results are discussed in relation to the ontogeny of beta-adrenoreceptors with the known noradrenergic innervation of the cerebellum, and to the paucity of both granule neurons and myelination which occurs in the neurological mutants.
用[3H]-二氢烯丙洛尔来检测织工鼠、旋转鼠、蹒跚鼠和颤搐鼠这几种神经学突变小鼠及其正常对照小鼠小脑β-肾上腺素能受体的发育情况。在正常动物中，[3H]结合的最大增加发生在出生后10至15天的时间段内，约3周时达到最大值。结合具有饱和性，表观解离常数为0.26 nM，并且[3]-二氢烯丙洛尔与其受体的亲和力在发育过程中没有显著变化。在20天时，织工鼠、旋转鼠和蹒跚鼠中[3H]-二氢烯丙洛尔结合显著降低（为对照值的14 - 22%），但颤搐突变体中没有。结合小脑已知的去甲肾上腺素能神经支配，以及神经学突变体中颗粒神经元和髓鞘形成的缺乏，对这些结果进行了讨论。
