Higuchi H, Takeyasu K, Watanabe Y, Uchida S, Yoshida H
Jpn J Pharmacol. 1982 Aug;32(4):671-8. doi: 10.1254/jjp.32.671.
Studies were made on developmental changes in phasic contractions of isolated rat vas deferens in response to norepinephrine (NE) and acetylcholine (ACh), which are mediated by alpha-adrenergic and muscarinic cholinergic receptors (alpha-R and m-R). The ED50 values of both contractions were almost constant during development, but the maximum responses to NE and ACh per wet weight and the relative contractions by NE and ACh to that by 100 mM K+ decreased greatly between 3 and 8 weeks after birth. However, the alpha- and m-receptors, assayed by measuring binding of [3H]-WB4101 and [3H]-quinuclidinyl benzylate (QNB), increased quantitatively per g wet weight and per mg protein between 2 and 8 weeks after birth, but did not change qualitatively. Thus, a discrepancy between the increases of alpha- and m-receptors and decreases of the maximum responses to NE and ACh was found, and possible mechanisms for this are discussed.
研究了去甲肾上腺素（NE）和乙酰胆碱（ACh）介导的离体大鼠输精管相性收缩的发育变化，它们分别由α-肾上腺素能受体和毒蕈碱型胆碱能受体（α-R和m-R）介导。在发育过程中，两种收缩的半数有效剂量（ED50）值几乎恒定，但出生后3至8周期间，每湿重对NE和ACh的最大反应以及NE和ACh相对于100 mM K+的相对收缩大幅下降。然而，通过测量[3H]-WB4101和[3H]-喹核醇基苯甲酸酯（QNB）的结合来测定的α-受体和m-受体，在出生后2至8周期间每克湿重和每毫克蛋白质的数量增加，但性质未变。因此，发现了α-受体和m-受体的增加与对NE和ACh的最大反应的降低之间存在差异，并讨论了其可能的机制。
