腺苷酸环化酶在大鼠口腔黏膜未固定标本及分离线粒体中的定位。

Adenylate cyclase localization in unfixed specimens of rat oral mucosa and isolated mitochondria.

作者信息

Fine A S, Egnor R W, Forrester E, Stahl S S

出版信息

J Histochem Cytochem. 1982 Nov;30(11):1171-8. doi: 10.1177/30.11.6292286.

DOI:10.1177/30.11.6292286

Abstract

The cytochemical localization of adenylate cyclase (E.C.4.6.1.1) was demonstrated in unfixed rat oral mucosal specimens and isolated mitochondrial fractions by use of a modified cytochemical reaction mixture. The adenylate cyclase reaction product was observed in the mitochondrial cristae of intact epithelial and fibroblast cells, and in isolated mitochondria after a 10-min incubation in the reaction mixture. The validity of the cytochemical adenylate cyclase method was supported by biochemical studies.

摘要

通过使用改良的细胞化学反应混合物，在未固定的大鼠口腔黏膜标本和分离的线粒体组分中证明了腺苷酸环化酶（E.C.4.6.1.1）的细胞化学定位。在完整的上皮细胞和成纤维细胞的线粒体嵴中，以及在反应混合物中孵育10分钟后的分离线粒体中，观察到了腺苷酸环化酶反应产物。生化研究支持了细胞化学腺苷酸环化酶方法的有效性。