1.71磅克/立方厘米牛卫星DNA的结构：与卫星I的进化关系。

Structure of 1.71 lb gm/cm(3) bovine satellite DNA: evolutionary relationship to satellite I.

作者信息

Taparowsky E J, Gerbi S A

出版信息

Nucleic Acids Res. 1982 Sep 25;10(18):5503-15. doi: 10.1093/nar/10.18.5503.

DOI:10.1093/nar/10.18.5503

PMID:6292843

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320891/

Abstract

The Eco RI fragments from the 2600 bp repeating unit of 1.711b gm/Cm(3) bovine satellite DNA were cloned in pBR322. The structure of the repeat unit was determined and compared to bovine satellite I DNA (rho CsCl = 1.715 gm/cm(3)). All of the DNA in the 1402 bp repeat of satellite I is represented in the sequence of the 2600 bp 1.711b gm/cm(3) repeat. The difference between the two repeats is due to a 1200 bp piece of DNA (INS) residing in the middle of the 1.711b gm/cm(3) repeat. The INS is AT-rich and has some repetitive components; it bears only limited similarity to the structure of eukaryotic transposable elements. We propose that the 1.711b gm/cm(3) satellite DNA arose via the amplification of a 1.715 gm/cm(3) satellite repeat altered by a 1200 bp insertion of DNA.

摘要

从密度为1.711克/立方厘米的牛卫星DNA的2600碱基对重复单元中获得的Eco RI片段被克隆到pBR322中。确定了重复单元的结构，并与牛卫星I DNA（氯化铯密度=1.715克/立方厘米）进行了比较。卫星I的1402碱基对重复序列中的所有DNA都存在于密度为1.711克/立方厘米的2600碱基对重复序列中。这两个重复序列的差异是由于一段1200碱基对的DNA片段（INS）位于密度为1.711克/立方厘米的重复序列中间。INS富含AT，有一些重复成分；它与真核转座元件的结构只有有限的相似性。我们提出，密度为1.711克/立方厘米的卫星DNA是通过插入1200碱基对DNA而改变的密度为1.715克/立方厘米的卫星重复序列的扩增产生的。