Yamada S, Matsuo K, Uchinuno Y
Avian Dis. 1982 Jul-Sep;26(3):596-601.
Specific-pathogen-free (SPF) ducks that were 1, 3, 4, 7, 10, 30, and 180 days old were inoculated experimentally orally or nasally with infectious bursal disease virus (IBDV). Attempts to induce clinical disease in ducks with strain J1 or FK-78 of IBDV were unsuccessful. Virus-recovery attempts from organ and intestinal contents were also unsuccessful. No significant gross or histopathological lesions were found in liver, spleen, kidney, heart, or bursa of Fabricius of 1- and 3-day-old ducks at 4 or 7 days postinoculation. The ratios of bursa weight to body weight of 1-, 10-, and 30-day-old inoculated and control ducks revealed no difference at 21 days postinoculation. The ducks responded serologically, however, by developing both virus-neutralizing and agar-gel-precipitin antibodies. Virus multiplied in embryonated duck eggs and duck embryo fibroblast cells but not in duck kidney cells.
选用1日龄、3日龄、4日龄、7日龄、10日龄、30日龄和180日龄的无特定病原体（SPF）鸭，通过口服或滴鼻方式对其进行传染性法氏囊病病毒（IBDV）的实验性接种。用IBDV J1株或FK - 78株感染鸭，未成功诱发鸭出现临床疾病。从器官和肠道内容物中回收病毒的尝试也未成功。在接种后4天或7天，1日龄和3日龄鸭的肝脏、脾脏、肾脏、心脏或法氏囊中未发现明显的大体或组织病理学病变。接种的1日龄、10日龄和30日龄鸭与对照鸭在接种后21天的法氏囊重量与体重之比无差异。然而，鸭通过产生病毒中和抗体和琼脂凝胶沉淀抗体做出了血清学反应。病毒能在鸭胚和鸭胚成纤维细胞中增殖，但不能在鸭肾细胞中增殖。
