Deutsch C, Slater L, Goldstein P
Biochim Biophys Acta. 1982 Nov 17;721(3):262-7. doi: 10.1016/0167-4889(82)90078-7.
Human peripheral blood lymphocytes exposed to hypotonic media (Ca/Mg-free, room temp.) first swell and then shrink. This shrinking response is characterized by a simple exponential with a half-time of 1.44 +/- 0.60 min (n = 11) and its extent but not the half-time for a given hypotonicity is influenced by [K+]0. Using K-selective electrodes, we observe a change in [K+]0 when cells are diluted into hypotonic media. A half-time of 1.55 +/- 0.06 min (n = 4) was obtained. A similar half-time was obtained by assay of total cell K using atomic absorption spectroscopy. At all osmolarities [K+]i was decreased from control values and was constant as [K+]0 was increased. Short-term incubation with ouabain (10(-4) M) had no effect. Decreasing osmolarities progressively inhibited phytohemagglutinin-stimulated DNA synthesis, yet cell number and viability remained unaltered. Our evidence indicates that the volume response is mediated by a change in the passive permeability of the plasma membrane to K and/or to the accompanying anions, and that the consequently volume-adapted cells are growth-inhibited.
暴露于低渗介质（无钙/镁，室温）中的人外周血淋巴细胞首先肿胀然后收缩。这种收缩反应的特征是简单指数形式，半衰期为1.44±0.60分钟（n = 11），并且对于给定的低渗度，其收缩程度而非半衰期受[K +] 0的影响。使用钾选择性电极，当细胞稀释到低渗介质中时，我们观察到[K +] 0的变化。得到的半衰期为1.55±0.06分钟（n = 4）。通过原子吸收光谱法测定总细胞钾也得到了类似的半衰期。在所有渗透压下，[K +] i均从对照值降低，并且随着[K +] 0的增加而保持恒定。用哇巴因（10（-4）M）短期孵育没有效果。渗透压的降低逐渐抑制了植物血凝素刺激的DNA合成，但细胞数量和活力保持不变。我们的证据表明，体积反应是由质膜对钾和/或伴随阴离子的被动通透性变化介导的，并且因此体积适应的细胞生长受到抑制。
