Wang G K, Strichartz G
Biophys J. 1982 Nov;40(2):175-9. doi: 10.1016/S0006-3495(82)84473-1.
The effects of purified scorpion toxins from two different species on the kinetics of sodium currents were evaluated in amphibian myelinated nerves under voltage clamp. A toxin from Leiurus quinquestriatus slowed and prevented sodium channel inactivation, exclusively, and a toxin from Centruroides sculpturatus Ewing reduced transient sodium currents during a maintained depolarization, and induced a novel inward current that appeared following repolarization, as previously reported by Cahalan (1975, J. Physiol. [Lond.]. 244:511-534) for the crude scorpion venom. Both of these effects were observed in fibers treated with both of these toxins, and the kinetics of the induced current were modified in a way that showed that the same sodium channels were modified simultaneously by both toxins. Although the toxins can act on different sites, the time course of the action of C. sculpturatus toxin was accelerated in the presence of the L. quinquestriatus toxin, indicating some form of interaction between the two toxin binding sites.
在电压钳制条件下，对两栖类有髓神经中两种不同种类的纯化蝎毒素对钠电流动力学的影响进行了评估。来自金蝎的一种毒素专门减缓并阻止钠通道失活，而来自尤氏杀人蝎的一种毒素在持续去极化期间降低瞬时钠电流，并诱导出一种复极化后出现的新型内向电流，这与之前卡哈兰（1975年，《生理学杂志》[伦敦]。244:511 - 534）对粗制蝎毒的报道一致。在用这两种毒素处理的纤维中均观察到了这两种效应，并且诱导电流的动力学发生了改变，表明相同的钠通道同时被两种毒素修饰。尽管毒素可以作用于不同位点，但在存在金蝎毒素的情况下，尤氏杀人蝎毒素的作用时间进程加快，这表明两个毒素结合位点之间存在某种形式的相互作用。
