Feige U, Overwien B, Sorg C
J Immunol Methods. 1982 Nov 12;54(3):309-15. doi: 10.1016/0022-1759(82)90315-5.
Leukocyte cell concentrates, obtained by continuous flow leukapheresis from single donors, were separated on a continuous hypotonic (260 mosM) Percoll gradient. On average, 86% of monocytes were recovered in a sharp band at a purity of up to 91% (average 76%). By this procedure 1-2 X 10(9) monocytes may be obtained from an individual donor. Hypotonic gradient purification, as compared with isotonic (295 mosM) conditions, proved superior with regard to capacity, speed of performance, yield and monocyte purity.
通过对单个供体进行连续流动白细胞单采术获得的白细胞浓缩物，在连续低渗（260 mosM）的Percoll梯度上进行分离。平均而言，86%的单核细胞在一个狭窄条带中回收，纯度高达91%（平均76%）。通过该程序，可从单个供体获得1-2×10⁹个单核细胞。与等渗（295 mosM）条件相比，低渗梯度纯化在处理能力、操作速度、产量和单核细胞纯度方面表现更优。
