Gorbunova L V, Varshaver N B, Marshak M I, Shapiro N I
Mol Gen Genet. 1982;187(3):473-6. doi: 10.1007/BF00332631.
The mutagenic activity of the tsA239 mutant of SV40 which synthetizes a defective T antigen at 40 degrees C was investigated in Chinese hamster cells under permissive and nonpermissive temperature. At 33 degrees C the virus increased the yield of 6-mercaptopurine-resistant colonies after 2 days expression time by a factor of 1.6-4 as compared with the control and raised the frequency of aberrant metaphases after the same time by a factor of 1.9-3.4. In the same experiments, with the same initially infected population of Chinese hamster cells, at 40 degrees C tsA SV40 did not induce either gene mutations or chromosome aberrations at the same early stage after infection. Presumably the activity of the A gene of SV40 is necessary not only for the transforming but also for the mutagenic effect of the virus.
研究了在允许温度和非允许温度下，SV40的tsA239突变体在中国仓鼠细胞中的诱变活性，该突变体在40℃时合成缺陷型T抗原。在33℃时，与对照相比，病毒在2天表达时间后使6-巯基嘌呤抗性菌落的产量增加了1.6至4倍，并在相同时间后使异常中期的频率增加了1.9至3.4倍。在相同实验中，对于相同初始感染的中国仓鼠细胞群体，在40℃时，tsA SV40在感染后的同一早期阶段既不诱导基因突变也不诱导染色体畸变。推测SV40的A基因的活性不仅对于病毒的转化作用而且对于诱变作用都是必需的。
