用聚乙烯吡咯烷酮-二氧化硅（Percoll）密度梯度分析犬外周血白细胞的浮力密度。

Analysis of buoyant density of canine peripheral blood leukocytes with PVP-Silica (Percoll) density gradients.

作者信息

Buurman W A, Vegt P A, Groenewegen G, van der Linden C J, Jeunhomme G M

出版信息

Vet Immunol Immunopathol. 1982 Nov;3(6):547-56. doi: 10.1016/0165-2427(82)90038-1.

Abstract

Isolation of canine peripheral blood mononuclear cells with a one step centrifugal separation procedure has not been very successful sofar. Significant contamination with polymorphonuclear cells has been reported. An analysis of the buoyant density of canine peripheral blood leukocytes on a self-generating Percoll gradient showed that the buoyant densities of polymorphonuclear cells and lymphocytes are so near that separation with high purity and yield is not possible with the use of a density gradient. Transient changes in buoyant density of polymorphonuclear cells have been observed. In such situations differences in buoyant density between cell types have been observed which permit separation of mononuclear cells from polymorphonuclear cells at a reasonable yield.

摘要

迄今为止，采用一步离心分离法分离犬外周血单个核细胞的效果并不理想。据报道，该方法会受到多形核细胞的严重污染。对犬外周血白细胞在自生成的 Percoll 梯度上的浮力密度进行分析后发现，多形核细胞和淋巴细胞的浮力密度非常接近，因此使用密度梯度无法实现高纯度、高产量的分离。已观察到多形核细胞浮力密度的短暂变化。在这种情况下，已观察到不同细胞类型之间浮力密度的差异，这使得能够以合理的产量从多形核细胞中分离出单核细胞。