[Isolation of a purified theta-hemolysin from C1. perfingens type A and its use for titrating toxins and sera].

Purified Cl. perfringens theta-hemolysin with a specific activity of 15-16 X 10(5) HU per mg of protein nitrogen, toxic for mice when injected intravenously, has been obtained by precipitation with zinc chloride, gel filtration through Sephadex G-100 and ion-exchange chromatography on DEAE Sephadex A-50. 1 Dcl of the toxin is 0.2-0.4 mg. The yield of theta-hemolysin amounting to 20-25% has been achieved, the degree of its purification reaching 80- to 120-fold. Equine anti-theta hemolytic serum has been obtained. The method for the determination of the anti-theta toxin-binding properties of Cl. perfringens toxins and the quantitative content of anti-theta-hemolysin in sera has been developed.