Carnegie J A, Tsang B K
Am J Obstet Gynecol. 1983 Jan 15;145(2):223-8. doi: 10.1016/0002-9378(83)90496-9.
The role of the calcium-calmodulin system in the gonadotropic regulation of ovarian steroidogenesis was studied by investigating the influence of various agents known to alter calcium metabolism or calmodulin activity on basal and follicle-stimulating hormone (FSH)-stimulated production of progesterone by rat granulosa cells in vitro. Lanthanum, a specific calcium antagonist, attenuated FSH-stimulated cyclic adenosine monophosphate (cyclic AMP) and progesterone production. [Ethylene-bis(oxyethylene-nitrilo)]tetraacetic acid (EGTA) significantly reduced this steroidogenic response but failed to alter the synthesis of the nucleotide. Although progesterone production was markedly increased by dibutyryl cyclic AMP, this was significantly lowered by an inhibitor of calcium uptake, verapamil. FSH-stimulated production of cyclic AMP and progesterone and dibutyryl cyclic AMP-induced progesterone biosynthesis were all significantly reduced by trifluoperazine, a specific inhibitor of calmodulin. These results indicate the existence of at least two sites in the trophic regulation of granulosa cell steroidogenesis which are calcium- and calmodulin-dependent: one localized at the level of cyclic AMP production, and the second at an unidentified step(s) distal to the formation of this nucleotide.
通过研究各种已知可改变钙代谢或钙调蛋白活性的试剂对体外培养的大鼠颗粒细胞基础和促卵泡激素（FSH）刺激的孕酮生成的影响，来探讨钙 - 钙调蛋白系统在卵巢甾体生成的促性腺调节中的作用。镧系元素，一种特定的钙拮抗剂，减弱了FSH刺激的环磷酸腺苷（cAMP）和孕酮的生成。乙二胺四乙酸（EGTA）显著降低了这种甾体生成反应，但未能改变核苷酸的合成。虽然二丁酰环磷酸腺苷显著增加了孕酮的生成，但钙摄取抑制剂维拉帕米使其显著降低。FSH刺激的环磷酸腺苷和孕酮的生成以及二丁酰环磷酸腺苷诱导的孕酮生物合成均被钙调蛋白的特异性抑制剂三氟拉嗪显著降低。这些结果表明，在颗粒细胞甾体生成的营养调节中至少存在两个钙和钙调蛋白依赖性位点：一个位于环磷酸腺苷生成水平，另一个位于该核苷酸形成远端的未确定步骤。
