商业用人白蛋白制剂与牛白蛋白制剂的比较研究。

A comparative study of commercial human and bovine albumin preparations.

作者信息

Farrance I, Dennis P M, Gibson B J, Biegler B

出版信息

Ann Clin Biochem. 1978 Jan;15(1):31-6. doi: 10.1177/000456327801500107.

Abstract

A number of commercial human and bovine albumin preparations were compared using seven assay procedures, to assess their suitability as reference materials for albumin and total protein assays. The results indicate that before a particular commercial albumin preparation can be used for standardisation purposes, its suitability should be checked in several assay systems which measure a different functional aspect of the protein molecule. The measurement of extinction coefficient in the range 278-280 nm does not appear to be a valid measure of protein content if the albumin preparation is to be used for standardisation of immunochemical or dye-binding assays.

摘要

使用七种检测方法对多种市售人源和牛源白蛋白制剂进行了比较，以评估它们作为白蛋白和总蛋白检测参考物质的适用性。结果表明，在将特定的市售白蛋白制剂用于标准化目的之前，应在多个检测系统中检查其适用性，这些系统测量蛋白质分子的不同功能方面。如果白蛋白制剂要用于免疫化学或染料结合检测的标准化，在278 - 280 nm范围内测量消光系数似乎不是蛋白质含量的有效测量方法。