Graziani Y, Erikson E, Erikson R L
J Biol Chem. 1983 Feb 25;258(4):2126-9.
This communication provides biochemical, immunological, and genetic evidence that pp60src, the Rous sarcoma virus transforming gene product, is associated with glycerol kinase activity. Our investigations demonstrated that the compound phosphorylated by pp60src or by glycerol kinase (EC 2.7.1.30) from Candida mycoderma share the same electrophoretic and chromatographic mobilities. The glycerol kinase and protein kinase activities of pp60src were inhibited similarly by preincubation with immune IgG. Both activities were reduced 6-9-fold in pp60src preparations derived by immunoaffinity chromatography from cells which were infected with NY68, a temperature-sensitive transformation mutant of Rous sarcoma virus. The thermolability at 41 degrees C of the glycerol kinase activity of pp60src from the mutant virus-infected cells was greater (t/2 = 1.3 min) than the same activity in pp60src preparations from wild type virus-infected cells (t/2 = 4.8 min).
本通讯提供了生物化学、免疫学及遗传学证据，表明劳氏肉瘤病毒转化基因产物pp60src与甘油激酶活性相关。我们的研究表明，由pp60src或来自皮状假丝酵母的甘油激酶（EC 2.7.1.30）磷酸化的化合物具有相同的电泳和色谱迁移率。pp60src的甘油激酶和蛋白激酶活性经与免疫IgG预孵育后受到类似抑制。通过免疫亲和色谱法从感染了劳氏肉瘤病毒温度敏感转化突变体NY68的细胞中获得的pp60src制剂中，这两种活性均降低了6至9倍。来自突变病毒感染细胞的pp60src的甘油激酶活性在41℃时的热稳定性（t/2 = 1.3分钟）比来自野生型病毒感染细胞的pp60src制剂中的相同活性（t/2 = 4.8分钟）更高。
