Khramov V A
Mikrobiologiia. 1982 Nov-Dec;51(6):1002-5.
The activity of carbamate kinase (EC 2.5.2.2) was determined in bacteria using a simple modified procedure. Carbamoyl phosphate produced under the action of carbamate kinase carbamoylated ammonia in a reaction which was not enzyme-catalyzed yielding urea that was assayed by the colorimetric technique. The activity of carbamate kinase was found by this method in a number of microorganisms. The method can be used to study other enzymes synthesizing carbamoyl phosphate. The advantages of the method over other techniques are discussed.
采用一种简单的改良方法测定了细菌中氨基甲酸激酶（EC 2.5.2.2）的活性。在氨基甲酸激酶作用下产生的氨甲酰磷酸使氨甲酰化，该反应不是酶催化的，生成的尿素通过比色技术进行测定。通过这种方法在许多微生物中发现了氨基甲酸激酶的活性。该方法可用于研究其他合成氨甲酰磷酸的酶。讨论了该方法相对于其他技术的优点。
