Godfrey P P, Lembra L, Coleman R
Biochem J. 1982 Oct 15;208(1):153-7. doi: 10.1042/bj2080153.
The microtubule poisons colchicine and vinblastine caused a reduction in the biliary output of total protein, compared with controls, in bile-fistula rats. The various protein components of bile showed patterns of change in output differing from one another. Alkaline phosphodiesterase I, a 'plasma-membrane' enzyme, showed a decline in output during the first hour after drug administration. Immunoglobulin A output did not decline until after the first hour. In contrast with these reductions, the biliary output of (rat) plasma albumin into bile was increased. At no time was there any evidence (as measured by lactate dehydrogenase release into bile) for any cytolytic damage. These results are discussed in relation to current theories on the output of proteins into bile and the repair of the plasma membrane in the bile canaliculus.
与对照组相比,微管毒素秋水仙碱和长春碱使胆瘘大鼠胆汁中总蛋白的胆汁排出量减少。胆汁的各种蛋白质成分显示出不同的排出量变化模式。“质膜”酶碱性磷酸二酯酶I在给药后第一小时内排出量下降。免疫球蛋白A的排出量直到第一小时后才下降。与这些减少情况相反,(大鼠)血浆白蛋白进入胆汁的胆汁排出量增加。在任何时候都没有证据(通过乳酸脱氢酶释放到胆汁中的量来衡量)表明存在任何细胞溶解损伤。结合目前关于蛋白质进入胆汁的输出以及胆小管质膜修复的理论对这些结果进行了讨论。