Barnwell S G, Coleman R
Biochem J. 1983 Nov 15;216(2):409-14. doi: 10.1042/bj2160409.
The microtubule poison, colchicine, caused an abnormal output of a variety of proteins into rat bile. After 3 h of exposure to the drug, livers were isolated and perfused with media of defined protein composition. There was no essential change in permeability of the hepatobiliary system to proteins (e.g. bovine serum albumin) entering bile from the perfusion fluid. The rat (serum) albumin and fibrinogen that were secreted into bile from colchicine-treated livers were probably derived from the hepatocytes. Disruption of the microtubular system reduces the secretion of proteins at the sinusoidal face of the hepatocyte and results in an accumulation of secretory vesicles in the cytoplasm. It is suggested that under these conditions some of the vesicles discharge their contents into the bile canaliculus.
微管毒物秋水仙碱可导致大鼠胆汁中多种蛋白质异常排出。在接触该药物3小时后，分离出肝脏并用蛋白质成分明确的培养基进行灌注。肝胆系统对从灌注液进入胆汁的蛋白质（如牛血清白蛋白）的通透性没有本质变化。从经秋水仙碱处理的肝脏分泌到胆汁中的大鼠（血清）白蛋白和纤维蛋白原可能来自肝细胞。微管系统的破坏会减少肝细胞窦状面蛋白质的分泌，并导致分泌小泡在细胞质中积累。有人认为，在这些情况下，一些小泡会将其内容物排入胆小管。
