Chung H R, Albanese E A, Studzinski G P
Cancer Res. 1983 Mar;43(3):1269-74.
Normal and simian virus 40-transformed human embryonic lung fibroblasts in culture were exposed to 3.5 microM coformycin (CF). This treatment resulted in almost complete inhibition of adenosine deaminase activity in both cell lines and retarded the progress of the fibroblasts through the cell cycle, as measured by the rate of cell proliferation. There was a marked difference, however, in the effect of CF on the traverse of different segments of the cell cycle. In normal fibroblasts, CF rapidly but transiently inhibited cell entry into visible stages of mitosis and delayed the progress through S and G2. In transformed fibroblasts, the effects of CF on the cell cycle included an early acceleration of the cell entry into visible mitosis and prolongation of this phase of the cell cycle. These results indicate that inhibition of adenosine deaminase can have different effects on the traverse of the critical segments of the cell cycle in normal and transformed cells.
将培养的正常人和经猿猴病毒40转化的人胚肺成纤维细胞暴露于3.5微摩尔的助间霉素（CF）中。这种处理几乎完全抑制了两种细胞系中的腺苷脱氨酶活性，并通过细胞增殖速率测量，减缓了成纤维细胞在细胞周期中的进程。然而，CF对细胞周期不同阶段进程的影响存在显著差异。在正常成纤维细胞中，CF迅速但短暂地抑制细胞进入有丝分裂的可见阶段，并延迟通过S期和G2期的进程。在转化的成纤维细胞中，CF对细胞周期的影响包括早期加速细胞进入可见的有丝分裂以及延长细胞周期的这一阶段。这些结果表明，抑制腺苷脱氨酶对正常细胞和转化细胞中细胞周期关键阶段的进程可产生不同影响。
